1. The development of tumor is often caused by the abnormal gene of multiple tumor hot spots in normal somatic cells.

2. Whether there is a hot spot mutation in the target detection sample, the early detection of the tumor, the molecular typing, and the target therapy are significant.

3. The genetic polymorphism and expression level of drug generation genes affect the concentration and sensitivity of the drug in the body, which leads to the individual difference in the drug reaction.

4. According to the detection of drug metabolizing enzymes and target genes, it has significant clinical significance to effectively guide the clinical individualized drug use.